Biodegradation of low density polyethylene with staphylococcussp. Isolated from the Ascension dump –Huancavelica

Abstract

The present investigation describes the isolation and biodegradative activity of the microorganism on low-density polyethylene (LPDE), with the objective of determining the degradation of low-density polyethylene by Staphylococcussp. The bacteria were isolated from LPDE with evidence of deterioration from the Ascensión-Huancavelica landfill. The collection of samples was carried out by observation, a sterile 250 ml bottle was used, transporting aseptically. The samples were homogenized in distilled water. Staphylococcussp.strains were isolated in salty Mannitol agar culture medium. The quantitative test of Staphylococcussp.for the samples was performed by Mac Farland No. 2. The degradation period of LPDE was 90 days in three simple convection ovens at temperatures of (37, 20 and 15°C). According to the results, the biodegradation of LPDE by the bacteria Staphylococcussp. Is observed at a temperature of 37°C, a degradation of 2,16%, at a temperature of 20 ° C, a degradation of 0.52% and finally at a temperature of 15 ° C a degradation of 0,29%, which means that at a temperature of 37 ° C Staphylococcus sp.is more efficient in terms of biodegradation and at 15 °C it is less efficient.