Revista Peruana de Ciencia Animal

COMPARISON OF TWO METHODS OF DNA EXTRACTION IN PARASITES (Sarcoptes scabiei) OF HUACAYA ALPACAS FROM THE PROVINCE OF HUANCAVELICA

2023-09-06T10:58:18+00:00

The objective of this work was to compare two DNA extraction methods (Phenol – Chloroform and Quick-DNA™ 96 Plus Kit) in parasites (Sarcoptes scabiei) of Huacaya alpacas in terms of quantity and quality (purity and integrity). For this, 18 alpacas were selected, from which samples of the skin lesions caused by scabies were extracted. Subsequently, the isolation of the parasites and the extraction of DNA were carried out with the two extraction methods mentioned. For data analysis, descriptive statistics and the Mann-Whitney U Test were used, using the R statistical program. Obtaining the following results: the amount of DNA for the Phenol – Chloroform (F-C) method was 6.4 ± 1.2 ng/ ul and for the Quick-DNA™ 96 Plus Kit (Quick) method it was 6.4 ± 1.6 ng/ul, not finding significant statistical differences (P>0.05), regarding the range of the DNA purity index (A260/A280 ), was between 1.8 ± 0.1 nm and 1.9 ± 0.1 nm for the F-C method and the Quick method respectively, not finding significant statistical differences (P>0.05); Regarding completeness, regular completenesses were observed in both methods. The amount of DNA obtained with the two extraction methods is small, but useful for molecular studies. As for quality, it is within the optimal range. Both extraction methods are similar with respect to the quantity and quality of the DNA.

EVALUATION OF FEEDING SYSTEMS AND QUANTITY OF FOOD ON PRODUCTIVE PARAMETERS OF GUINEA PIGS (Cavia porcellus)

2023-09-06T11:05:21+00:00

The objective of this work was to evaluate feeding systems and amount of food on the productive parameters of guinea pigs (Cavia porcellus). For this, 90 weaned male guinea pigs of the Peru breed were used, randomly distributed in nine treatments. Weight gain, feed intake and feed conversion were determined. The weight gain with respect to the feeding systems was: 663.97 g for the mixed feeding, 412.66 g for the feeding based on only hydroponic green fodder (FVH) of oats - vetch and 398.48 g for the feeding based on balanced food ; The results of the statistical analysis present significant statistical differences (P<0.05) between systems, registering the highest weight gain in the mixed system. The weight gain with respect to the amount of food was: 669.52 g, 527.46 g and 278.13 g for C1 (ad libitum), C2 (250 g of FVH of oats - vetch and 50 g of balanced food) and C3 (200 g of FVH of oats - vetch and 20 g of balanced food) respectively; The results of the statistical analysis present significant statistical differences (P<0.05) between amounts of food, obtaining the highest weight gain with C1. The food consumption regarding the feeding systems of the guinea pigs was: 3,864.07 g for the mixed feeding, 3,500.72 g for the feeding based on only oat-vetch FVH and 1,698.65 g for the balanced feeding; The results of the statistical analysis present significant statistical differences (P<0.05) between systems, registering the highest food consumption in the mixed system. The food consumption regarding the amount of food was: 4,114.57 g, 2,941.25 g and 2,007.63 g for C1, C2 and C3 respectively; The results of the statistical analysis present significant statistical differences (P<0.05) between amounts of food, registering the highest food consumption in C1. The feed conversion regarding the feeding systems was: 5.22 for the feed based on balanced feed, 5.81 for the mixed feed, and 9.79 for the feed based on only FVH of oats - vetch; The results of the statistical analysis present significant statistical differences (P<0.05) between systems, registering the best feed conversion in the balanced system. The feed conversion with respect to the amount of food was: 5.53, 6.33 and 8.96 for C2, C1 and C3 respectively; The results of the statistical analysis show significant statistical differences (P<0.05) between amounts of feed, obtaining the best feed conversions in C2 and C1. The feeding systems and the amounts of food have an effect on the productive parameters of guinea pigs of the Peru breed.

EVALUATION OF PRODUCTIVE AND CHEMICAL PARAMETERS OF THREE VARIETIES OF FORAGE OATS ASSOCIATED WITH VICIA

2023-09-06T23:21:52+00:00

The present study was carried out with the objective of evaluating the productive (Plant Height "AP", Nº of Plants per m2 "NPM2", Green Matter Yield "RMV" and Dry Matter Yield "RMS") and chemicals (Dry Matter "DM", Crude Protein "PC", Ethereal Extract "EE", Neutral Detergent Fiber "NDF", Acid Detergent Fiber "FDA" and Ash "C") of three varieties of fodder oats associated with Vicia sativa in two types of sowing. To evaluate the productive parameters, a Completely Random Design with a 3x2 Factorial Arrangement was used (being the factors evaluated varieties of oats and types of sowing) and to evaluate the chemical parameters a Completely Random Design was used (being the factor evaluated only varieties of oats); The treatments to evaluate the productive parameters constituted the following combinations: Avena mantaro 15 + Vicia sativa in rows (T1), Avena mantaro 15 + Vicia sativa in broadcast (T2), Avena strigosa + Vicia sativa in rows (T3), Avena strigosa + Vicia sativa in broadcast (T4), Avena cayuse + Vicia sativa in rows (T5) and Avena cayuse + Vicia sativa in broadcast ( T6). There was no significant interaction between the oat variety factor and the type of sowing regarding the productive parameters; the variety factor had a significant influence (P<0.05) only in AP of oats and vetch; In the same way, the type of planting had a significant influence (P<0.05) only on Vetch NPM2; on the other hand, no significant statistical differences (P>0.05) were found in RMV and RMS with respect to variety and type of planting. Finally, no significant statistical differences (P>0.05) were found in any of the chemical parameters (DM, PC, EE, NDF, FDA and C) with respect to the oat variety.

GENETIC VARIABILITY IN TWO POPULATIONS OF LLAMAS (Lama glama) IN HUANCAVELICA

2023-09-07T00:26:04+00:00

The present study was carried out with the purpose of laying the foundations to undertake a program of genetic improvement or conservation of llamas, for this reason the objective was to determine the genetic variability in two populations of llamas in the Huancavelica region. For which, DNA samples from 44 randomly chosen llamas were studied. These samples were processed using a panel of 14 fluorolabeled microsatellite markers, these were subsequently amplified and then separated by capillary electrophoresis with the ABI 3130XL automatic sequencer. A total of 172 alleles were found in the study populations. In the population of the Center for Research and Development of South American Camelids - Lachocc (CIDCS - Lachocc), the number of alleles per locus (Na) was 90 in total, in a range of 3 to 11 alleles, the effective number of alleles ( Ne) was 46.9 in total, with ranges from 1.62 to 5.38; the observed (Ho) and expected (He) heterozygosity with ranges from 0.88 to 0.29 and 0.81 to 0.39, respectively. In the Lircay population, the Na was 82 in total, in a range of 3 to 8 alleles, the Ne was 43.51 in total, with ranges from 1.23 to 5.16; Ho and He with ranges from 0.95 to 0.2 and 0.81 to 0.19, respectively. The average polymorphic information content (PIC) for the two populations was 0.65, with ranges from 0.39 to 0.80. The inbreeding coefficient (Fis) for the CIDCS - Lachocc showed low levels of inbreeding (-0.0075) in the same way for the Lircay population (-0.0145). The Fst values were 0.074 and Fit 0.064, indicating a moderate genetic differentiation. Concluding that there is a moderate genetic variability in these populations.

TECHNOLOGICAL CHARACTERISTICS OF THE LLAMA FIBER (Lama glama) BEFORE AND AFTER DEHAIRING

2023-09-07T00:43:47+00:00

The present investigation was carried out at the Center for Research and Development of South American Camelids - Lachocc (CIDCS - Lachocc) and in the Textile Fibers Area of the Genetic Improvement Laboratory of the National University of Huancavelica with the objective of evaluating the technological characteristics of the fiber of Chaku llamas, when dehaired and without dehairing, the fiber samples were obtained from the average rack of 40 llamas. For the analysis of the fiber samples, the OFDA 2000 equipment was used, with the characteristics studied being the average fiber diameter (MDF), the coefficient of variation of the MDF (CVMDF) and the comfort factor (FC). Data were processed using descriptive statistics and Student's T test for related samples. The results show that, by subjecting the fibers to the manual dehairing process, fine fibers of good textile quality are obtained, since statistically significant differences (P<0.05) were found between the technological characteristics of the fiber before and after dehairing; demonstrating the decrease of the MDF by 12.03 μm, the CVDMF by 16.89% and increasing the FC by 39.73%. It is concluded that the dehairing process is an alternative to improve the most important aspects of the technological characteristics of the fiber, achieving greater fineness, comfort and less variation.

Complete number

2023-09-21T01:16:32+00:00

Editorial

2023-09-20T11:01:45+00:00